Contents

 

Introduction

The Infrared Microspectroscopy (IRM) beamline combines the high brilliance and high collimation of the synchrotron beam with a Bruker V80v Fourier transform infrared (FTIR) spectrometer and a Hyperion 2000 IR microscope to reach high signal-to-noise ratios at diffraction limited spatial resolutions; between 3-8 μm. This makes the beamline ideally suited to the analysis of microscopic samples e.g. small particles and thin layers within complex matrices, or thin coatings on surfaces. In addition, a Hyperion 3000 Focal Plane Array (FPA) FTIR microscope is also installed offline at the beamline. This allows users to collect large area overview IR images from their samples prior to high resolution mapping using the beamline instrument.

Each microscope can be operated in the following modes of data collection (see Samples for more information) and can be coupled to a number of different accessories to assist with the wide variety of experiments conducted at the beamline:

  • Transmission
  • Reflectance
  • Grazing Incidence
  • microAttenuated Total Reflectance (ATR)

In each of the above modes a motorised stage allows the point-by-point collection (raster mapping) of individual spectra from a sample, to create spatially resolved “spectral maps” (see Techniques Available for more information).

Figure 1. The IR Microscopy Cabin.

 

 Figure 2 The Bruker V80v FT-IR spectrometer and Hyperion 2000 IR Microscope  
Figure 2. The Bruker V80v FT-IR spectrometer and Hyperion 2000 IR Microscope. Figure 3. The Bruker Hyperion 3000 Focal Plane Array microscope.

Each microscope is a combined IR/white-light microscope, to allow visible images of each sample to be collected and areas of interest highlighted for IR analysis: Köhler apertures and fluorescence illumination (excitation @480nm; emission@520nm) are available to assist with sample contrast.

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Applications

The following list highlights some of the areas of research studied so far using the IRM beamline:
  •         Treatments for Multiple Sclerosis
  •         Effect of diabetes drugs
  •         Improved in vitro fertilisation
  •         Stem-cell therapy
  •         Fingerprint analysis
  •         Art & document conservation
  •         Biocompatible coatings for materials
  •         Anti-fouling coatings
  •         Water purification membranes
  •         Chemistry of ancient volcanic rocks
  •         Oil-bearing minerals
  •         Effects of climate change
  •         Biominerals in marine snails
  •         Photovoltaic coatings
  •         Malaria treatments
  •         Organic content of farmed soils
  •         Degradable plastics
  •         Inkjet inks 

Combined IR transmission studies & x-ray microspectroscopy

It is possible to combine IR transmission studies with x-ray microscopy on the X-ray Fluorescence Microprobe beamline. Please contact a beamline scientist for more information.

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